Electronic Theses and Dissertation

Silverskin merupakan salah satu produk sampingan pengolahan kopi yang biasanya dibuang dan menjadi limbah. Tujuan dari penelitian ini adalah untuk mengetahui potensi ekstrak silverskin kopi arabika (Coffea arabica L.) Gayo sebagai antioksidan. Penelitian ini adalah penelitian eksperimental dimana ekstrak silverskin kopi arabika Gayo dilakukan beberapa pemeriksaan yaitu uji aktivitas antioksidan dengan metode DPPH (2,2-diphenyl-1-picrylhydrazyl), uji total fenolik dan tanin dengan metode Folin-Ciocalteu, uji total flavonoid dengan metode kolorimetri AlCl3 dan pemeriksaan GC-MS (Gas Chromatography-Mass Spectrometry). Hasil uji aktivitas antioksidan ekstrak silverskin kopi arabika Gayo menunjukkan nilai IC50 sebesar 8.28 ppm dan vitamin C sebagai kontrol positif menunjukkan nilai IC50 sebesar 2.25 ppm. Hasil ini menunjukkan ekstrak silverskin kopi arabika Gayo memiliki aktivitas antioksidan yang sangat kuat. Ekstrak silverskin kopi arabika Gayo memiliki nilai total fenolik sebesar 9.4 mg GAE/g, nilai total flavonoid sebesar 40.4 mg QE/g, dan nilai total tanin sebesar 40.4 mg TAE/g. Hasil dari pemeriksaan GC-MS terdapat 39 komponen senyawa, namun hanya 14 senyawa yang teridentifikasi sebagai antioksidan yaitu Trans-β-caryophyllene (0.13%); Butylated Hidroxy Toluene (0.43%); Benzophenone (0.12%); 2-Pentadecanone, 6,10,14-trimethyl- (0.32%); Caffeine (1.85%); 9-Octadecenoic acid (Z)- (CAS) Oleic acid (2.63%); Hexadecanoic acid, ethyl ester (18.80%); Oleic acid (1.46%); Ethyl cis, cis-9,12-octadecadienoate (5.27%), Ethyl linoleate (0.22%), Linoleic acid ethyl ester (0.28%), 9,12-Octadecadienoic acid (Z,Z)-ethyl ester (0.11%); Glycerol 1,3- Dihexadecanoate (0.79%)dan Hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester (1.87%). Ekstrak silverskin kopi arabika Gayo berpotensi sebagai antioksidan sehingga dapat dimanfaatkan dalam mencegah penyakit degeneratif.

Silverskin is a by-product of coffee processing which is usually thrown away and becomes waste. The aim of this research is to determine the potential of Gayo arabica coffee (Coffea arabica L.) silverskin extract as an antioxidant. This is an experimental research in which Gayo arabica coffee silverskin extract was subjected to several tests, namely antioxidant activity test using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method, total phenolic and tannin test using the Folin-Ciocalteu method, total flavonoid test using the colorimetric AlCl3 method and GC-MS (Gas Chromatography-Mass Spectrometry) examination. The results of the antioxidant activity test showed an IC50 value of 8.28 ppm and vitamin C as a positive control showed an IC50 value of 2.25 ppm. These results show that silverskin extract has very strong antioxidant activity. This silverskin extract has a total phenolic value of 9.4 mg GAE/g, a total flavonoid value of 40.4 mg QE/g, and a total tannin value of 40.4 mg TAE/g. The results of the GC-MS examination contained 39 compound components, but only 14 compounds were identified as antioxidants, namely Trans-β-caryophyllene (0.13%); Butylated Hydroxy Toluene (0.43%); Benzophenone (0.12%); 2-Pentadecanone, 6,10,14-trimethyl- (0.32%); Caffeine (1.85%); 9-Octadecenoic acid (Z)-(CAS) Oleic acid (2.63%); Hexadecanoic acid, ethyl ester (18.80%); Oleic acid (1.46%); Ethyl cis, cis-9,12-octadecadienoate (5.27%), Ethyl linoleate (0.22%), Linoleic acid ethyl ester (0.28%), 9,12-Octadecadienoic acid (Z,Z)-ethyl ester (0.11%); Glycerol 1,3-Dihexadecanoate (0.79%) and Hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester (1.87%). Gayo arabica coffee silverskin extract has the potential as an antioxidant so it can be used to prevent degenerative diseases.