Electronic Theses and Dissertation

Kayu manis (Cinnamomum burmanii) dan pala (Myristica fragrans Houtt) merupakan tumbuhan yang sering digunakan dalam berbagai pengobatan tradisional salah satunya untuk menangani diabetes mellitus. Pada pengembangan obat herbal selain pertimbangan efikasi, sangat penting dinilai tingkat toksisitas herbal tersebut. Penelitian ini bertujuan untuk mengetahui kandungan metabolit sekunder dan membandingkan aktivitas inhibisi α glukosidase serta uji toksisitas ekstrak kayu manis dan pala Aceh. Kedua ekstrak diekstraksi menggunakan metode maserasi dengan pelarut etanol 70%. Uji aktivitas antidiabetes dengan inhibisi α glukosidase secara in vitro menggunakan metode spektrofotometri UV-Vis dengan panjang gelombang 410 nm. Akarbose digunakan sebagai pembanding. Sedangkan untuk mengetahui toksisitasnya dilakukan dengan metode Brine Shrimp Lethality Test (BSLT) sehingga diperoleh nilai LC50. Hasil analisa kandungan metabolit sekunder ekstrak kayu manis mengandung alkaloid, flavonoid, polifenol, tannin, kuinon, saponin, dan triterpenoid, sedangkan ekstrak pala mengandung saponin, tanin, flavonoid, steroid. Kadar flavonoid, tanin, fenol total ekstrak kayu manis adalah 80.52 mg QE/gr, 66.34 mg GAE/gr, 566.33 mg AT/gr. Kadar flavonoid, tanin, fenol total ekstrak pala adalah 144,26 mg QE/gr, 64,65 mg GAE/gr, 8,24 mg AT/gr. Hasil pengukuran aktivitas inhibisi α glukosidase menunjukkan bahwa nilai IC50 ekstrak kayu manis dan pala Aceh adalah 123.65 μg/mL dan 125.84 μg/mL. Sedangkan hasil penelitian uji toksisitas menunjukan bahwa nilai LC50 ekstrak kayu dan pala Aceh adalah 350.57 µg/mL dan 161.62 µg/mL. Ekstrak kayu manis dan pala Aceh dikategorikan sebagai ekstrak yang memiliki toksisitas sedang dengan aktivitas antidiabetes yang sedang.

Kata kunci: α glukosidase, toksisitas, kayu manis, pala, antidiabetes

Cinnamon (Cinnamomum burmanii) and nutmeg (Myristica fragrans Houtt) are plants that are often used in various traditional medicines, one of which is to treat diabetes mellitus. In the development of herbal medicines apart from efficacy considerations, it is very important to assess the level of toxicity of these herbs. This study aims to determine the content of secondary metabolites and to compare the inhibitory activity of α-glucosidase and the toxicity test of Acehnese cinnamon and nutmeg extracts. Both extracts were extracted using maceration method with 70% ethanol solvent. Test antidiabetic activity with inhibition α glucosidase in vitro using UV-Vis spectrophotometry method with a wave length of 410 nm. Acarbose was used as a comparison. Meanwhile, to determine the toxicity is done by the method Brine Shrimp Lethality Test (BSLT) so that the value is obtained LC50. The results of analysis of the secondary metabolite content of cinnamon extract contain alkaloids, flavonoids, polyphenols, tannins, quinones, saponins, and triterpenoids, while nutmeg extract contains saponins, tannins, flavonoids, steroids. Flavonoid, tannin, total phenolic content of cinnamon extract were 80.52 mg QE/gr, 66.34 mg GAE/gr, 566.33 mg AT/gr. Flavonoid, tannin, total phenolic levels of nutmeg extract were 144.26 mg QE/gr, 64.65 mg GAE/gr, 8.24 mg AT/gr. The results of measuring the activity of α glucosidase inhibition showed that IC50 value Acehnese cinnamon and nutmeg extracts are123.65μg/mL and 125.84 μg/mL. Meanwhile, the results of the toxicity test showed LC50 values of Aceh wood and nutmeg extracts were 350.57 µg/mL and 161.62 µg/mL. Aceh cinnamon and nutmeg extracts are categorized as extracts that have moderate toxicity with moderate antidiabetic activity. Keywords: α glucosidase, toxicity, cinnamon, nutmeg, antidiabetic