Electronic Theses and Dissertation

Diabetes melitus (DM) menyebabkan gangguan fungsi motorik usus dalam penyerapan zat gizi. Pemberian probiotik dan tanaman herbal dapat menjaga saluran cerna, flora normal usus, barrier epitel, dan inhibitor  glukosidase dengan membatasi mikroorganisme patogen dan meningkatkan sekresi musin oleh sel goblet. Penelitian ini bertujuan mengetahui pemberian susu kambing fermentasi L. casei dan filtrat pandan terhadap sebaran karbohidrat dan histomorfometri usus halus tikus model DM. Penelitian ini adalah penelitian eksperimental laboratorium Rancangan Acak Lengkap (RAL). Sebanyak 24 ekor tikus jantan dikelompokkan 6 perlakuan dan 3 ulangan yaitu K- (tikus normal), K+ (STZ 30 mg/kg BB/i.p), P1 (metformin dosis 60 mg/kg BB (p.o)), P2 (susu kambing fermentasi dan filtrat pandan (2 ml/p.o)). P3 (susu kambing fermentasi (2 ml/p.o), P4 (filtrat pandan (2 ml/p.o), selama 14 hari. Tikus putih dinekropsi dengan prosedur euthanasia secara cervical dislocation, lalu sampel usus halus dikoleksi dan kemudian diwarnai dengan pewarnaan histokimia menggunakan alcian blue (AB) mengekspresikan karbohidrat bersifat asam, periodic acid Schiff (PAS) mengekspresikan karbohidrat bersifat netral, pengukuran histomorfometri untuk mengetahui rataan lebar usus halus, serta menghitung jumlah dan diameter sel goblet. Hasil penelitian pada K-, K+, P1, P2, P3, dan P4 menunjukkan variasi sebaran karbohidrat asam maupun netral. Pengukuran rataan histomorfometri usus halus tikus menunjukkan adanya perbedaan nyata (P

Diabetes mellitus (DM) causes disturbances in intestinal motor function in the absorption of nutrients. Probiotics and herbal plants can maintain the gastrointestinal tract, normal intestinal flora, epithelial barrier, and α-glucosidase inhibitors by limiting pathogenic microorganisms and increasing mucin secretion by goblet cells. This study aims to determine the administration of L. casei fermented goat's milk and pandan filtrate on the distribution of carbohydrates and histomorphometry of the small intestine of DM model rats. This research is a Completely Randomized Design (CRD) laboratory experimental research. A total of 24 male rats were grouped into 6 treatments and 3 replications, namely K- (normal rats), K+ (STZ 30 mg/kg BW/i.p), P1 (metformin dose 60 mg/kg BW (p.o)), P2 (fermented goat's milk and pandan filtrate (2 ml/p.o)). P3 (fermented goat's milk (2 ml/p.o), P4 (pandan filtrate (2 ml/p.o), for 14 days. White rats were necropsied using a cervical dislocation euthanasia procedure, then small intestine samples were collected and then stained with histochemical staining using alcian blue (AB) expresses acidic carbohydrates, periodic acid Schiff (PAS) expresses neutral carbohydrates, histomorphometric measurements to determine the average width of the small intestine, and calculate the number and diameter of goblet cells. Results of research on K-, K+, P1, P2, P3 , and P4 shows variations in the distribution of acidic and neutral carbohydrates. Measurement of the average histomorphometry of the small intestine of rats showed a significant difference (P