Electronic Theses and Dissertation

UJI SENSITIVITAS KLORAMFENIKOL, STREPTOMISIN, DAN AZTREONAM MENGHAMBAT PERTUMBUHAN BAKTERI
Vibrio sp. YANG DIISOLASI DARI IKAN LELE DUMBO
(Clarias gariepinus)

ABSTRAK
Penggunaan antibiotik sebagai growth promoter sering dicampurkan langsung di dalam pakan ternak ikan lele dumbo (Clarias gariepinus) yang pemberian dalam waktu lama dan berlebihan dapat menyebabkan residu antibiotik dan resistensi bakteri. Penelitian ini bertujuan untuk mengetahui aktivitas daya hambat kloramfenikol, streptomisin, dan aztreonam dalam menghambat pertumbuhan bakteri Vibrio sp. pada ikan lele dumbo (C. gariepinus). Penelitian ini menggunakan stok isolat bakteri Vibrio sp. yang diisolasi dari ikan lele dumbo oleh peneliti sebelumnya yang disimpan di Laboratorium Mikrobiologi. Penelitian dilakukan dengan tahap pembiakan stok isolat bakteri dalam media Nutrient Broth (NB), direidentifikasi dalam media Thiosulfate Citrate Bile Salt Sucrose (TCBS) dan pewarnaan Gram. Selanjutnya dilakukan uji sensitivitas antibiotik kloramfenikol, streptomisin, dan aztreonam di media Muelller Hinton Agar (MHA). Hasil penelitian memperlihatkan adanya pertumbuhan bakteri di media NB. Pertumbuhan bakteri juga terlihat di media TCBS yang secara pewarnaan Gram menunjukkan bakteri Gram negatif berbentuk batang bengkok dan berwarna merah. Hasil uji sensitivitas menunjukkan zona hambat kloramfenikol dengan diameter 16,2 mm, streptomisin dengan diameter 16,5 mm, dan aztreonam dengan diameter 21,1 mm. Dapat disimpulkan bahwa kloramfenikol memiliki aktivitas daya hambat intermediet dan streptomisin serta aztreonam sensitif terhadap pertumbuhan bakteri Vibrio sp. yang diisolasi dari ikan lele dumbo (C. gariepinus).

Kata kunci: Aztreonam, Clarias gariepinus, Kloramfenikol, Streptomisin, Vibrio

THE SENSITIVITY TEST OF CLORAMPHENICOL, STREPTOMYCIN, AND AZTREONAM INHIBITING GROWTH OF Vibrio sp. BACTERIA ISOLATED FROM DUMBO CATFISH (Clarias gariepinus) ABSTRACT The use of antibiotics as growth promoters is often mixed directly in African catfish (Clarias gariepinus) feed where prolonged and inordinate can cause antibiotic residues and bacterial resistance. This study aims to determine the inhibitory activity of chloramphenicol, streptomycin, and aztreonam in inhibiting the growth of Vibrio sp. in African catfish (C. gariepinus). This study used Vibrio sp. bacterial stock isolates. which was isolated from African catfish by previous researchers kept in the Microbiology Laboratory. The research was carried out by culturing stock of bacterial isolates in Nutrient Broth (NB) medium, re-identification in Thiosulfate Citrate Bile Salt Sucrose (TCBS) medium and Gram staining. Furthermore, the antibiotic sensitivity test for chloramphenicol, streptomycin, and aztreonam was carried out on Mueller Hinton Agar (MHA) media. The results showed that there was bacterial growth on NB media. Bacterial growth was also seen in TCBS media which in Gram stain showed Gram negative bacteria in the form of bent rods and red in color. The sensitivity test results showed inhibition zones for chloramphenicol with a diameter of 16.2 mm, streptomycin with a diameter of 16.5 mm, and aztreonam with a diameter of 21.1 mm. It can be concluded that chloramphenicol has intermediate inhibitory activity and streptomycin and aztreonam are sensitive to the growth of Vibrio sp. isolated from African catfish (C. gariepinus). Keywords: Aztreonam, Clarias gariepinus, Chloramphenicol, Streptomycin, Vibrio