Electronic Theses and Dissertation

Diabetes mellitus (DM) merupakan suatu kondisi medis di mana terjadi peningkatan kadar glukosa dalam darah yang disebabkan karena ketidakmampuan tubuh dalam mengatur kadar glukosa. Salah satu efek DM adalah penurunan fungsi organ reproduksi jantan berupa penurunan kualitas spermatozoa dan pada akhirnya dapat menyebabkan infertilitas. Penelitian bertujuan bertujuan untuk mengetahui pengaruh pemberian madu kelulut terhadap peningkatan kualitas spermatozoa tikus putih diabetes mellitus. Dua puluh lima ekor tikus putih jantan dengan bobot badan 200 - 250 gram yang dibagi lima perlakuan yaitu 5 perlakuan dengan 5 kali pengulangan pada tiap perlakuan. Perlakuan 1 sebagai kontrol negatif (KN) yaitu tikus normal yang tidak diinduksi 120 mg/kgBB streptozotocin dan madu kelulut namun hanya diberikan pakan standar selama 50 hari. Perlakuan 2 sebagai kontrol positif (KP) yaitu tikus yang diinduksi 120 mg/kgBB streptozotocin selama 7 hari tanpa diberi madu kelulut. Perlakuan 3 sebagai perlakuan 1 (P1) yaitu tikus yang diinduksi 120 mg/kgBB streptozotocin selama 7 hari dan diberi madu kelulut dosis 10 mg/kgBB/hari. Perlakuan 4 sebagai perlakuan 2 (P2) yaitu tikus yang di induksi 120 mg/kgBB streptozotocin selama 7 hari dan diberi madu kelulut dosis 50 mg/kgBB/hari. Perlakuan 5 sebagai perlakuan 3 (P3) yaitu tikus yang diinduksi 120 mg/kgBB streptozotocin selama 7 hari dan diberi madu kelulut dosis 100 mg/kgBB/hari. Perlakuan pemberian madu kelulut secara keseluruhan dilakukan selama 50 hari. Tikus dieuthanasia dengan cara cervical dislocation kemudian dinekropsi. Koleksi spermatozoa diambil dari organ cauda epididimis. Kualitas spermatozoa diamati terhadap konsentrasi, motilitas dan viabilitas spermatozoa. Data dianalisis menggunakan analisis of varians, dan dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan perlakuan pemberian madu kelulut dapat meningkatkan secara nyata (P

Diabetes mellitus (DM) is a medical condition in which there is an increase in blood glucose levels caused by the body's inability to regulate glucose levels. The dangerous effect of DM is a decrease in the function of the male reproductive organs, namely the testes, resulting in a decrease in the quality of spermatozoa, and can ultimately cause infertility. The aim of this study was to determine the effect of giving kelulut honey on improving the quality of spermatozoa of white rats with diabetes mellitus. Twenty five male white rats with a body weight of 200 - 250 grams were divided into five treatments, namely 5 treatments with 5 repetitions for each treatment. Treatment 1 as a negative control (KN), namely normal rats that were not induced by 120 mg/kgBW of streptozotocin and kelulut honey but only given standard feed for 50 days. Treatment 2 was a positive control (KP), namely rats induced by 120 mg/kg BW streptozotocin for 7 days without being given kelulut honey. Treatment 3 as treatment 1 (P1), namely mice that were induced by 120 mg/kgBW streptozotocin for 7 days and given kelulut honey at a dose of 10 mg/kgBW/day. Treatment 4 as treatment 2 (P2), namely mice that were induced by 120 mg/kgBW streptozotocin for 7 days and given kelulut honey at a dose of 50 mg/kgBW/day. Treatment 5 as treatment 3 (P3), namely mice that were induced by 120 mg/kgBW streptozotocin for 7 days and given kelulut honey at a dose of 100 mg/kgBW/day. The overall treatment of kelulut honey was carried out for 50 days. Mice were euthanized by cervical dislocation and then necropsied. Spermatozoa collection was taken from the cauda epididymis organ. The quality of spermatozoa was observed on the concentration, motility and viability of spermatozoa. Data were analyzed using analysis of variance, and continued with Duncan's test. The results showed that the treatment with kelulut honey was significantly different (P