Electronic Theses and Dissertation

Aeromonas hydrophila merupakan bakteri yang menyebabkan penyakit Motile
Aeromonas Septicemia. Penyakit ini biasanya dikendalikan dengan menggunakan antibiotik akan
tetapi dapat mengakibatkan dampak negatif seperti bakteri menjadi resisten, oleh sebab itu
digunakan alternatif lain dengan menggunakan bahan alami seperti daun malaka (Phyllanthus
emblica). Dalam kandungannya, daun malaka diduga dapat menghambat pertumbuhan bakteri.
Penelitian ini bertujuan untuk mengetahui adanya aktivitas antibakteri ektrak etanol daun malaka
(Phyllanthus emblica) terhadap pertumbuhan bakteri Aeromonas hydrophila ATTC 7966.
Penelitian ini menggunakan metode Kirby-Bauer (disc diffusion) dengan 3 konsentrasi (25%, 50%,
75%) ekstrak etanol daun malaka, kontrol positif digunakan kloramfenikol dan kontrol negatif
digunakan aquades. Reidentifikasi bakteri dilakukan dengan pewarnaan Gram dan uji aktivitas
antibakteri dilakukan pada media MHA (Muller Hinton Agar). Rata-rata diameter zona hambat
yang terbentuk pada masing-masing konsentrasi adalah 25% (14,88 mm), 50% (17,04 mm) dan
75% (18,51 mm. Dapat disimpulkan bahwa ekstrak etanol daun malaka (Phyllanthus emblica)
memiliki aktivitas daya hambat pertumbuhan bakteri Aeromonas hydrophila (ATTC 7966).

Aeromonas hydrophila is a bacterium that causes Motile Aeromonas Septicemia. This disease is usually controlled by using antibiotics but can have negative impacts such as bacteria becoming resistant, therefore another alternative is used using natural ingredients such as Malacca leaves (Phyllanthus emblica). In its content, Malacca leaves are thought to inhibit the growth of bacteria. This study aims to determine the antibacterial activity of the ethanol extract of Malacca leaves (Phyllanthus emblica) on the growth of Aeromonas hydrophila ATTC 7966. This study used the Kirby-Bauer method (disc diffusion) with 3 concentrations (25%, 50%, 75%) of ethanol extract Malacca leaves, the positive control used chloramphenicol and the negative control used distilled water. Re-identification of bacteria was carried out by Gram staining and antibacterial activity test was carried out on MHA media (Muller Hinton Agar). The average diameter of the inhibition zone formed at each concentration was 25% (14.88 mm), 50% (17.04 mm) and 75% (18.51 mm. It can be concluded that the ethanol extract of Malacca leaves (Phyllanthus emblica) has the activity of inhibiting the growth of Aeromonas hydrophila bacteria (ATTC 7966).