Electronic Theses and Dissertation

Viperin merupakan protein yang diinduksi oleh interferon yang berfungsi menghambat replikasi berbagai virus, salah satunya virus Newcastle disease (VND). Ayam merupakan unggas yang paling rentan terhadap infeksi VND, dibandingkan dengan itik. Penelitian ini bertujuan untuk melihat ekspresi dan perbedaan persentase area sel imunopositif protein terhadap viperin pada otak ayam kampung dan itik pasca diinfeksikan isolat VND virulen asal Aceh. Sampel penelitian berupa otak ayam kampung dan itik dari 4 kelompok percobaan yaitu AC-A (kelompok ayam kampung yang diinfeksikan VND), AC-I (kelompok itik yang diinfeksikan VND), K-A (kelompok kontrol ayam kampung yang diinokulasikan PBS) dan K-I (kelompok kontrol itik yang diinokulasikan PBS). Sampel organ dikoleksi pada hari ke-1, 3 dan 7 pasca infeksi (pi). Penelitian ini menggunakan virus isolat Ducks/Aceh Besar_IND/2013/eoAC080721 yang diisolasi dari daerah Lambaro, Aceh Besar. Nekropsi untuk koleksi organ dilakukan setiap hari pengamatan masing-masing 3 ekor per kelompok. Ekspresi protein viperin dideteksi dengan pewarnaan imunohistokimia menggunakan primary antibody Anti-Viperin Ea (1:250, Thermo-PA523508). Data yang diperoleh dari hasil pewarnaan imunohistokimia dianalisis secara deskriptif dan dihitung persentase area sel imunopositif terhadap protein viperin menggunakan ImageJ. Hasil penelitian menunjukkan protein viperin terekspresi pada sitoplasma sel glia, neuropil dan sedikit di sel neuron pada otak ayam kampung dan itik. Pada hari ke-1 pi persentase area sel imuopositif terhadap protein pada otak ayam kampung sama dengan itik. Pada hari ke-3 dan 7 pi persentase area sel imunopositif pada otak itik lebih tinggi dibandingkan dengan ayam kampung. Berdasarkan hasil penelitian tersebut disimpulkan bahwa terdapat ekspresi protein viperin dan persentase area sel imunopositif terhadap protein viperin pada otak itik lebih tinggi daripada ayam kampung.

Kata kunci: Ayam kampung, itik, Newcastle disease, pewarnaan imunohistokimia, viperin

Viperin is a protein induced by interferon that inhibits the replication of various viruses, including Newcastle disease virus (NDV). Chickens are the most susceptible to NDV infection, compared to ducks. This study aims to look at the expression and differences in the percentage of immunopositive cell areas of viperin protein in the brains of domestic chickens and ducks after being infected with virulent NDV isolates from Aceh. The research samples were brains of domestic chickens and ducks from 4 experimental groups, namely AC-A (domestic chicken group infected with NDV), AC-I (duck group infected with NDV), K-A (domestic chicken control group inoculated with PBS) and K-I (duck control group inoculated with PBS). Organ samples were collected on days 1, 3 and 7 post-infection (pi). This study used virus isolate Ducks/Aceh Besar_IND/2013/eoAC080721 isolated from Lambaro, Aceh Besar. Necropsy for organ collection was performed every day of observation, with 9 birds per group. Viperin protein expression was detected by immunohistochemical staining using primary antibody Anti-Viperin Ea (1:250, Thermo-PA523508). Data obtained from immunohistochemical staining results were analyzed descriptively and the percentage of immunopositive cell area against viperin protein was calculated using ImageJ. The results showed that viperin protein was expressed in the cytoplasm of glial cells, neurophil and a few neuron cells in the brain of domestic chickens and ducks. On day 1 pi, the percentage of protein-immunopositive cell areas in the brain of domestic chickens was similar to that of ducks. On days 3 and 7 pi the percentage of immunopositive cell areas in the duck brain was higher than that of domestic chickens. Based on the results of this study, it is concluded that there is an expression of viperin protein and the percentage of immunopositive cell areas to viperin protein in the brain of ducks is higher than that of domestic chickens. Keywords: Domestic chicken, duck, immunohistochemical staining, Newcastle disease, viperin