Electronic Theses and Dissertation

Bakteri endofit adalah bakteri yang hidup bersimbiosis dengan tanaman tanpa merusak jaringan tanaman inangnya. Penelitian ini bertujuan untuk mengetahui potensi bakteri endofit asal daun kari (Murraya koenigii (L.) Spreng.) sebagai antibakteri dan untuk mengetahui nilai Minimum Inhibitory Concentration (MIC) dalam menghambat pertumbuhan bakteri. Metode penelitian yang digunakan untuk menguji potensi antibakteri adalah metode difusi agar. Hasil penelitian menunjukkan bahwa isolat yang berhasil diisolasi dari daun kari berjumlah sebanyak sembilan isolat yang diberi kode BEDK1, BEDK2, BEDK3, BEDK4, BEDK5, BEDK6, BEDK7, BEDK8, dan BEDK 9. Isolat yang memiliki kemampuan sebagai antibakteri yaitu isolat BEDK6 dan BEDK8. Isolat BEDK6 memiliki nilai rata-rata diameter zona hambat terhadap pertumbuhan Escherichia coli sebesar 13,5 mm. Isolat BEDK8 memiliki nilai rata-rata zona hambat terhadap pertumbuhan Streptococcus mutans sebesar 4,4 mm dan Pseudomonas aeruginosa sebesar 3,5 mm. Nilai Minimum Inhibitory Concentration (MIC) isolat BEDK6 terhadap bakteri Escherichia coli berada pada konsentrasi 3,12% dengan nilai absorbansi sebesar 0,734.

Kata kunci: bakteri endofit, daun kari, antibakteri, Minimum Inhibitory Concentration (MIC)

Endophytic bacteria are bacteria that live in symbiosis with plants without damaging the host plant tissue. This study aims to determine the potency of endophytic bacteria from curry leaves (Murraya koenigii (L.) Spreng.) as an antibacterial and to determine the Minimum Inhibitory Concentration (MIC) value in inhibiting bacterial growth. The research method used to test the antibacterial potential is the agar diffusion method. The results showed that nine isolates that were successfully isolated from curry leaves were coded BEDK1, BEDK2, BEDK3, BEDK4, BEDK5, BEDK6, BEDK7, BEDK8, and BEDK9. The isolates that had antibacterial abilities were isolates BEDK6 and BEDK8. BEDK6 isolates had an average diameter of the inhibition zone on Escherichia coli growth of 13.5 mm. BEDK8 isolate has an average value of inhibition zone on the growth of Streptococcus mutans of 4.4 mm and Pseudomonas aeruginosa of 3.5 mm. The Minimum Inhibitory Concentration (MIC) value of BEDK6 isolate for Escherichia coli bacteria was at a concentration of 3.12% with an absorbance value of 0.734. Keywords: endophytic bacteria, curry leaves, antibacterial, Minimum Inhibitory Concentration (MIC)