Electronic Theses and Dissertation

PENGARUH PEMBERIAN EKSTRAK UMBI TUMBUHAN SARANG SEMUT ACEH (Hydnophytum formicarum)
TERHADAP PERUBAHAN KADAR KOLESTEROL
TIKUS PUTIH JANTAN (Rattus norvegicus)
DIABETES MELITUS TIPE-2



ABSTRAK

Hiperkolesterolemia merupakan keadaan meningkatnya konsentrasi kolesterol dalam darah yang melebihi nilai normal. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak tumbuhan sarang semut (Hydnophytum formicarum) terhadap perubahan kadar kolesterol tikus putih jantan (Rattus norvegicus) diabetes melitus. Penelitian ini menggunakan sampel sebanyak 25 ekor tikus putih jantan, sehat, berumur 3-4 bulan dengan berat badan antara 150-200 gram. Tikus dibagi menjadi 5 kelompok perlakuan. Kelompok P1 sebanyak 5 ekor diberi pakan biasa dan air, sedangkan kelompok P2, P3, P4, dan P5 diinjeksi aloksan secara intraperitoneal dosis tunggal 120 mg/kg bb serta diberi pakan tinggi lemak dan dibiarkan selama 14 hari untuk membuat tikus diabetes melitus. Pada hari ke 22 dilakukan pemeriksaan kadar kolesterol dengan easy touch GCU. Tikus kelompok P3 diberi Metformin dengan dosis 50 mg/kg bb, untuk kelompok P4 dan P5 diberi perlakuan terapi ekstrak tumbuhan sarang semut dengan dosis 100 mg/kg bb dan 200 mg/kg bb. Pemberian terapi dilakukan selama 14 hari secara oral dua kali sehari. Parameter yang diamati adalah perubahan kadar kolesterol pada tikus putih jantan. Analisis data yang digunakan ialah statistik One way ANOVA dengan uji lanjutan Duncan. Hasil analisis statistik menunjukkan bahwa pemberian ekstrak tumbuhan sarang semut berpengaruh nyata (P

THE EFFECT OF GIVING ACEH ANT NEST PLANT (Hydnophytum formicarum) TUBERS EXTRACT ON CHANGES IN CHOLESTEROL LEVELS MALE WHITE RATS (Rattus norvegicus) TYPE-2 DIABETES MELLITUS ABSTRACT Hypercholesterolemia is a state of increased cholesterol concentration in the blood that exceeds normal values. This study aims to determine the effect of giving ant nest plant extract (Hydnophytum formicarum) on changes in cholesterol levels of white male rats (Rattus norvegicus) with diabetes mellitus. This study used a sample of 25 male white rats, healthy, aged 3-4 months, weighing between 150-200 grams. Mice were divided into 5 treatment groups. In Group P1 as many as 5 animals were given regular feed and water. At the same time, groups P2, P3, P4, and P5 were injected with alloxan intraperitoneally in a single dose of 120 mg/kg bw and given high-fat feed and left for 14 days to make rats with diabetes mellitus. On the 22nd day, cholesterol levels were checked using the GCU easy touch. The P3 treatment rats were given Metformin at a dose of 50 mg/kg body weight, for the P4 and P5 treatments were treated with ant nest plant extract therapy at 100 mg/kg body weight and 200 mg/kg body weight. Therapy was given for 14 days orally twice a day. The parameters observed were changes in cholesterol levels in white male rats. The data analysis used was One way ANOVA statistics with Duncan's follow-up test. The statistical analysis showed that administering ant nest plant extract had a real effect (P