Electronic Theses and Dissertation

Tujuan penelitian ini untuk mengevaluasi metabolit sekunder ekstrak etanol daun kersen (Muntingia calabura L.) secara kualitatif melalui uji antioksidan, uji fitokimia, analisis GC-MS serta analisis FT-IR, mengevaluasi senyawa yang terdapat pada daun kersen dan berpotensi dalam penyembuh luka disebabkan oleh Diabetes Mellitus, mengevaluasi pada fase inflamasi dan proliferatif serta maturasi, dan mengevaluasi formulasi serta konsentrasi krim yang sesuai untuk penyembuhan luka disebabkan oleh Diabetes Mellitus.
Hewan uji yang digunakan yaitu 45 ekor tikus putih (Rattus novergicus). Sampel daun Kersen diperoleh dari pertanian lokal di sekitar area Banda Aceh pad koordinat 5032’23‖N 95020’06‖E 9,75 km. Kelompok 1 hewan diabetes hanya mendapat base krim 2x (Kontrol negatif). Kelompok 2 hewan diabetes menerima silver sulfadiazine (SSD). Kelompok 3 hewan penderita diabetes mendapat base krim daun Kersen 5%. Kelompok 4 hewan penderita diabetes mendapat base krim daun Kersen 10%. Kelompok 5 hewan penderita diabetes mendapat base krim daun Kersen 15 %, semua di berikan 2x sehari selama 14 hari. Perawatan luka Diabetes Mellitus pagi hari pukul 08.00 WIB dan sore hari pukul 18.00 WIB. Pada hari ke 3, 7, dan 14 tikus dieuthanasi untuk untuk koleksi sampel jaringan luka kulit. Histopatologi dengan pewarnaan Hemaktosilin Eosin (HE).
Dari hasil uji pemakian krim diketahui bahwa sediaan krim ekstrak etanol daun kersen 10% dan krim ekstrak etanol daun kersen 15% mempercepat proses inflamasi, epitelisasi dan sintesis kolagen sehingga mempercepat penyembuhan luka diabetes mellitus kulit tikus (P

The purpose of this study was to evaluate the secondary metabolites of cherry leaf ethanol extract (Muntingia calabura L.) qualitatively through antioxidant tests, phytochemical tests, GC-MS analysis and FT-IR analysis, evaluate compounds contained in cherry leaves and potentially in wound healing caused by Diabetes Mellitus, evaluate in inflammatory and proliferative phases and maturation, and evaluate formulations and cream concentrations that are appropriate for wound healing caused by Diabetes Mellitus. The test animals used were 45 white rats (Rattus novergicus). Kersen leaf samples were obtained from local farms around the Banda Aceh area at coordinates 5032'23‖N 95020'06‖E 9.75 km. Group 1 diabetic animals only got 2x base cream (negative control). Group 2 diabetic animals received silver sulfadiazine (SSD). Group 3 diabetic animals got a 5% Kersen leaf cream base. Group 4 diabetic animals got a 10% Kersen leaf cream base. Group 5 diabetic animals got 15% Kersen leaf base cream, all given 2x a day for 14 days. Diabetes Mellitus wound care in the morning at 08.00 WIB and in the afternoon at 18.00 WIB. On days 3, 7, and 14 rats were euthanized for collection of skin wound tissue samples. Histopathology with Hemaktocillin Eosin (HE) staining. From the results of the cream cursing test, it is known that the preparation of 10% cherry leaf ethanol extract cream and 15% cherry leaf ethanol extract cream accelerates the inflammatory process, epithelialization and collagen synthesis so as to accelerate the healing of rat skin diabetes mellitus wounds (P