Electronic Theses and Dissertation

Escherichia coli merupakan flora normal alami pada tubuh manusia dan hewan, namun ada beberapa strain patogen dan membawa gen virulen. Penularan penyakit dapat terjadi akibat mengonsumsi makanan yang sudah terkontaminasi E. coli patogen. Penelitian ini bertujuan mengetahui adanya cemaran E. coli patogen pada ceker ayam pedas yang dijual di Kecamatan Syiah Kuala. Penelitian ini menggunakan metode observasi dengan pendekatan Cross sectional study. Pada isolasi bakteri dikerjakan sesuai dengan metode Carter. Sepuluh sampel ceker ayam pedas dikoleksi dari berbagai pedagang di Kecamatan Syiah Kuala. E. coli diisolasi dan diidentifikasi dengan media Eosin Methylene Blue Agar (EMBA) dan Pewarnaan Gram. Koloni bakteri yang tumbuh diinokulasi ke media selektif Sorbitol MacConkey Agar (SMAC). Hasil penelitian didapatkan tujuh dari sepuluh sampel ceker ayam pedas yang diteliti positif terdapat E. coli patogen. Oleh sebab itu, dapat disimpulkan bahwa dari sepuluh sampel yang diperiksa terdapat tujuh sampel positif (70%) dapat diisolasi E. coli patogen pada ceker ayam pedas yang dijual di Kecamatan Syiah Kuala, Kota Banda Aceh.

Escherichia coli is a natural normal flora in the human body and animals, but there are several strains that are pathogenic and carry virulent genes. The transmission of disease can occur due to consuming food that has been contaminated with pathogenic E. coli. This study aims to determine the presence of pathogenic E. coli contamination in spicy chicken feet sell in the District of Syiah Kuala. This research used the observation method with a cross sectional study approach. Bacterial isolation was carried out according to the Carter’s method. Ten samples of spicy chicken feet were collected from various traders in the Syiah Kuala District. E. coli was isolated and identified with Eosin Methylene Blue Agar (EMBA) medium and Gram staining. Growing bacterial colonies were inoculated onto Sorbitol MacConkey Agar (SMAC) selective media. The results showed that seven out of ten samples of the spicy chicken claws tested were positive for pathogenic E. coli. Therefore, it can be concluded that of ten samples examined, there were seven positive samples of pathogenic E. coli (70%) can be isolated in spicy chicken feet sold in Syiah Kuala District, Banda Aceh.