Electronic Theses and Dissertation

Daun srikaya (Annona squamosal L.) diekstraksi dengan metanol sehingga diperoleh ekstrak metanol. Ekstrak metanol dipartisi dengan n-heksana sehingga diperoleh ekstrak n-heksana. Ekstrak metanol diekstraksi kembali dengan etil asetat sehingga diperoleh ekstrak etil asetat. Ekstrak etil asetat dipisahkan komponen kimia-kimianya dengan kromatografi gravitasi menggunakan fase diam silika gel G60 (70-230 mesh) dan fase gerak n-heksana:etil asetat (elusi gradien), diperoleh empat fraksi gabungan (ASE 1-ASE 4). ASE 3 mempunyai aktivitas antioksidan yang kuat sehingga selanjutnya dire-kromatografi menggunakan fase gerak n-heksana:etil asetat (elusi gradien) diperoleh empat fraksi gabungan (ASE 3.1-ASE 3.4). ASE 3.4 direkromatografi sampai diperoleh isolat murni (ASE 3.4.4). Uji aktivitas antioksidan dengan metode DPPH terhadap ASE 3.4.4 diperoleh nilai IC50 84,2 ppm dengan IC50 dari asam askorbat sebesar 6,65 ppm, uji toksisitas dengan metode BSLT terhadap ASE 3.4.4 diperoleh nilai LC50 22,85 ppm dan uji antikanker terhadap sel MCF-7 dengan metode MTT assay diperoleh nilai IC50 1,81 ppm. Berdasarkan hasil pengujian antioksidan, toksisitas dan antikanker dapat diketahui isolat ASE 3.4.4 sangat potensial sebagai bahan baku obat. Komponen aktif yang terdapat pada ASE 3.4.4 berdasarkan data GC-MS adalah 9-Octadecenoic acid (Z)-, methyl ester.

Kata kunci : Annona squamosa L, ekstrak etil asetat, antioksidan, sitotoksik dan antikanker

Srikaya (Annona squamosa) leaves were extracted with methanol to obtain methanol extract. The methanol extract was partitioned with n-hexane to obtain n-hexane extract. The methanol extract was extracted again with ethyl acetate to obtain ethyl acetate extract. The chemical components of the ethyl acetate extract were separated by gravity chromatography using silica gel 60G stationary phase and n-hexane:ethyl acetate as mobile phase (gradient elution), four combined fractions were obtained (ASE 1- ASE 4). ASE 3 has strong antioxidant activity so that it was then re-chromatographed using the mobile phase n-hexane:ethyl acetate (gradient elution) to obtain four combined fractions (ASE 3.1- ASE 3.4). ASE 3.4 was rechromatographed until pure isolate was obtained (ASE 3.4.4). Antioxidant activity test using the DPPH method against ASE 3.4.4 obtained an IC50 value of 84.2 ppm with IC50 of ascorbic acid is 6,65 ppm, toxicity test using the BSLT method for ASE 3.4.4 obtained an LC50 value of 22.85 ppm and anticancer test on MCF-7 cells using the MTT assay method obtained an IC50 value of 1.81 ppm. Based on the result of antioxidant, toxicity and anticancer tests, it was found that ASE 3.4.4 isolate had great potential as a raw material for medicine. Based on GC-MS data, the active component contained in ASE 3.4.4 is 9-Octadecenoic acid (Z)-, methyl ester. Kata kunci : Annona squamosa L, ethyl acetate extract, antioxidant, cytotoxic and anticancer