Electronic Theses and Dissertation

Nama : Deffan Dericco
Program Studi : Pendidikan Dokter Gigi
Judul Skripsi : Aktivitas Antibiofilm Susu Kambing terhadap Biofilm
Streptococcus mutans dan Candida albicans pada Jaringan
Lunak Mulut

Streptococcus mutans dan Candida albicans sebagai penyebab karies gigi membentuk biofilm di dalam rongga mulut. Biofilm merupakan bentuk agregat multiseluler dari sel bakteri maupun jamur sebagai bentuk pertahanan diri terhadap antimikroba dan perubahan lingkungan. Salah satu upaya untuk mencegah terjadinya karies gigi adalah dengan menghambat pembentukan biofilm melalui pemberian bahan antibakterial. Susu kambing mengandung laktoferin, laktoperoksidase, dan lisozim bersifat antibakteri dan antioksidan. Penelitian ini bertujuan untuk mengetahui aktivitas antibiofilm susu kambing terhadap kedua jenis mikroba tersebut pada jaringan lunak mulut. Penelitian ini dilakukan secara in vitro untuk mengukur konsentrasi massa biofilm menggunakan perhitungan Biofilm Coverage Rate (BCR) dan Biofilm Assay. Selain itu juga dilakukan pengamatan profil protein biofilm secara in vivo pada tikus Wistar yang dimanipulasi karies. Tikus yang telah mengalami karies dibagi menjadi 3 kelompok, yaitu kelompok perlakuan yang diberikan susu kambing, kelompok kontrol positif amoxicillin dan kelompok kontrol negatif diberikan akuades. Hasil penelitian menunjukkan kelompok perlakuan, kontrol negatif, dan kontrol positif masing-masing memiliki profil protein dengan rentang 41-67 kDA, 65,4-72,5 kDA, dan 33-34,1 kDA. Hasil tersebut menunjukkan bahwa profil protein pada biofilm tikus pada kelompok perlakuan mengalami penurunan nilai kDA walaupun hasil kontrol positif lebih baik demikian juga dengan hasil perhitungan konsentrasi BCR dan Biofilm Assay. Hasil analisis statistik dengan menggunakan uji oneway ANOVA menunjukkan bahwa pemberian susu kambing berpengaruh terhadap biofilm Streptococcus mutans dan Candida albicans (p=0,000). Disimpulkan bahwa susu kambing memiliki aktivitas antibiofilm susu kambing terhadap biofilm Streptococcus mutans dan Candida albicans pada jaringan lunak mulut.

Kata kunci: biofilm, Streptococcus mutans, Candida albicans, Biofilm Coverage Rate (BCR), profil protein

Name : Deffan Dericco Study Program : Dentistry Title : Antibiofilm activity of goat milk against Streptococcus mutans and Candida albicans biofilms in the mouth soft tissues Streptococcus mutans and Candida albicans form biofilms in the oral cavity as a cause of dental caries. A biofilm is a form of the multicellular aggregate of bacterial and fungal cells as self-defense against antimicrobials and environmental changes. One of the ways to prevent dental caries is by inhibiting the formation of biofilms through the treatment of antibacterial agents. Goat milk contains lactoferrin, lactoperoxidase, and lysozyme, which have antibacterial and antioxidant properties. This study aims to determine the antibiofilm activity of goat's milk against both types of microbes in the mouth's soft tissues. This research was conducted in vitro to measure the concentration of biofilm mass using the calculation of the Biofilm Coverage Rate (BCR) and Biofilm Assay. Observation of biofilm protein profiles was also carried out in vivo caries-manipulated Wistar rats. Rats with caries were divided into three groups: the treatment group was given goat's milk, the positive control group was given amoxicillin, and the negative control group was given aquadest. The results showed that the treatment group, negative control, and positive control each had a protein profile in the range of 41-67 kDA, 65.4-72.5 kDA, and 33-34.1 kDA. These results indicated that the protein profile in the rat biofilm in the treatment group decreased in kDA. However, the positive control results were better, as well as the results of calculating the concentration of BCR and Biofilm Assay. Statistical analysis using the one-way ANOVA test showed that goat's milk affected Streptococcus mutans and Candida albicans (p= 0.000). It was concluded that goat milk has antibiofilm activity against Streptococcus mutans and Candida albicans in the mouth's soft tissues. Key Word: biofilm, Streptococcus mutans, Candida albicans, Biofilm Coverage Rate (BCR), protein profile