Electronic Theses and Dissertation

ABSTRAK
Torsio testis terjadi ketika korda spermatika terpelintir yang memotong suplai darah ke testis sehingga terjadi kondisi iskemik dan peningkatan Reaktif Oksidase Spesies (ROS) yang menyebabkan penurunan kualitas sperma dan peningkatan Actin Binding Protein (ABP) sehingga dapat mengakibatkan infertilitas. Daun kelor (Moringa oleifera L) memiliki kandungan antioksidan yang tinggi dan dapat digunakan dalam penatalaksanaan infertilitas. Tujuan penelitian untuk mengetahui pengaruh pemberian daun kelor terhadap kualitas sperma dan actin binding protein (ABP) testis pada tikus Rattus novergicus akibat torsio testis. Jenis penelitian adalah post test only control group design. Setelah dilakukan aklimatisasi selama 2 minggu, dilakukan perlakuan, torsio testis dan diberikan ekstrak daun kelor 200 mg, 400 mg, dan 800 mg kemudian dilakukan analisis sperma dan pemeriksaan ABP testis. Analisis data perbedaan antar kelompok dilakukan dengan uji Analisis of variance (ANNOVA). Hasil didapatkan pada dosis 800 mg kualitas sperma dan titer ABP tikus lebih tinggi dibandingkan kelompok Dosis 400 mg dan kelompok dosis 200 mg. Kesimpulan didapatkan terdapat pengaruh pemberian ekstrak daun kelor (Moringa Oleifera) terhadap kualitas spermatozoa dan titer ABP pada torsio testis tikus (Rattus Novergicus).

Kata kunci : Torsio testis, Daun kelor, kualitas sperma, actin binding protein.

ABSTRACT Testicular torsion occurs when the spermatic cord is twisted which cuts off the blood supply to the testicles resulting in ischemic conditions and an increase in Reactive Oxidase Species (ROS) which causes a decrease in sperm quality and an increase in Actin Binding Protein (ABP) that can result in infertility. Moringa leaves (Moringa oleifera L) have a high antioxidant content and can be used in the management of infertility. The aim of the study was to determine the effect of giving Moringa leaves on sperm quality and testicular actin binding protein (ABP) in Rattus novergicus rats due to testicular torsion. This type of research is a post test only control group design. After acclimatization for 2 weeks, the treatment was carried out, testicular torsion and given 200 mg, 400 mg, and 800 mg of moringa leaf extract, then sperm analysis and testicular ABP examination were carried out. Data analysis of differences between groups was carried out using the Analysis of Variance (ANNOVA) test. The results obtained at a dose of 800 mg sperm quality and ABP titers of rats were higher than the 400 mg dose group and the 200 mg dose group. The conclusion was that there was an effect of giving Moringa leaf extract (Moringa Oleifera) on the quality of spermatozoa and ABP titers in testicular torsion of rats (Rattus Novergicus). Keywords: Testicular torsion, Moringa leaves, sperm quality, actin binding protein.