Electronic Theses and Dissertation

ANALISIS KERAGAMAN GEN KALPAIN-1 (CAPN1) PADA
DOMBA LOKAL

ABSTRAK

Domba lokal memiliki karakteristik badan yang kecil dan bulu berwarna kecoklatan. Keempukan daging domba dipengaruhi oleh beberapa faktor, salah satunya adalah faktor genetik. Gen yang mengontrol keempukan daging setelah disembelih adalah gen kalpain-1 (CAPN1). Tujuan penelitian ini untuk mengetahui keragaman gen CAPN1 pada domba lokal. Penelitian dilakukan menggunakan metode polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) dan dianalisis dengan hukum Hardy-Weinberg. Hasil produk amplifikasi PCR berada pada ukuran ̃̃~320 pb. Hasil RFLP deoxyribonucleic acid (DNA) diperoleh dua pola pemotongan fragmen DNA yaitu, tiga pemotongan pita DNA pada sampel 2 hingga sampel 10 dan dua pemotongan pita DNA pada sampel 1. Pemotongan pita DNA untuk sampel 2 hingga sampel 10, yaitu ~320 pb, ~210 pb dan ~110 pb serta pemotongan pita DNA pada sampel satu, yaitu ~210 pb dan ~110 pb. Hasil tersebut mengindikasikan bahwa keragaman gen CAPN1 pada domba lokal adalah polimorfik. Frekuesi genotip CC, CG, GG pada domba lokal yaitu 0, 0,10, dan 0,90, sedangkan frekuensi alel yang didapat adalah C sebesar 0,05 dan G sebesar 0,95. Hal ini mengindikasikan bahwa terjadi mutasi genetik pada gen CAPN1 pada domba lokal. Dapat disimpulkan bahwa terdapat keragaman gen CAPN1 pada domba lokal asal Kabupaten Bireuen, meskipun masih tergolong rendah.

Kata Kunci: Gen CAPN1, keempukan daging, marka genetik, PCR-RFLP

ANALYSIS OF CALPAIN-1(CAPN1) GENE POLYMORFISM IN LOCAL SHEEP ABSTRACT Local sheep have the characteristics of a small body and brown fur. The tenderness of lamb is influenced by several factors, one of which is genetic factors. The gene that controls the tenderness of meat after slaughter is the calpain-1 gene (CAPN1). The purpose of this study was to determine the diversity of the CAPN1 gene in local sheep. The study was conducted using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and analyzed using the Hardy-Weinberg law. The results of PCR amplification products were in the size of ~320 bp. The results of RFLP deoxyribonucleic acid (DNA) obtained two patterns of cutting DNA fragments, namely, three cutting DNA bands in sample 2 to sample 10 and two cutting DNA bands in sample 1. DNA band cutting for sample 2 to sample 10, which is ~320 bp, ~ 210 bp, and ~110 bp, and DNA band cutting in sample one, namely ~210 bp and ~110 bp. These results indicate that the diversity of the CAPN1 gene in local sheep is polymorphic. The genotype frequencies of CC, CG, and GG in local sheep were 0, 0.10, and 0.90, while the allele frequencies obtained were 0.05 for C and 0.95 for G. This indicates that there is a genetic mutation in the CAPN1 gene in local sheep. It can be concluded that there is a diversity of CAPN1 genes in local sheep from Bireuen Regency, although it is still relatively low. Keywords: CAPN1 gene, genetic markers, meat tenderness, PCR-RFLP