Electronic Theses and Dissertation

ABSTRAK
Telah dilakukan biosintesis nanopartikel tembaga dengan metode green synthesis menggunakan ekstrak air akar golodokan tiang (Polyalthia longifolia) sebagai bioreduktor dan stabilisator. Hasil pemeriksaan Total Phenolic Content (TPC) metode Folin-Ciocalteu adalah 85,43 GAE/g dan hasil pemeriksaan Total Flavonoid Content (TFC) adalah 0,78 QE/g. Kandungan phenolic dan flavonoid yang terdapat pada ekstrak sampel memiliki potensi untuk menjadi agen pereduksi dan stabilisasi yang bertanggung jawab untuk pembentukan nanopartikel tembaga. Hasil reaksi reduksi ekstrak sampel terhadap ion Cu2+ dikonfirmasi dengan menggunakan Spektrofotometer UV-Vis, Fourier Transform Infrared (FTIR), X-Ray Diffraction (XRD), Scanning Electron Microscope-Energy Dispersive X-Ray (SEM-EDX) dan Transmission Electron Microscopy (TEM). Nanopartikel tembaga yang terbentuk menunjukkan aktivitas antimikroba yang baik terhadap bakteri Staphylococcus aureus, bakteri Escherichia coli dan jamur Candida albicans. Aktivitas antibakteri dari hasil zona hambat pada konsentrasi 20% dikategorikankuat (resistant)dan pada antijamur dikategorikan sedang (intermediate).

Kata kunci: Nanopartikel tembaga (Cu), aktivitas antimikroba, ekstrak air, Polyalthia longifolia.

ABSTRACT The biosynthesis of copper nanoparticles using the green synthesis method has been carried out using the aqueous extract of the glodokan root (Polyalthia longifolia) as a bioreductor and stabilizer. The results of the examination of Total Phenolic Content (TPC) using the Folin-Ciocalteu method and the Total Flavonoid Content (TFC) were 85.43 GAE/g nm and 0.78 QE/g respectively. The phenolic and flavonoid content contained in the extract has the potential to be a reducing and stabilizing agent that is responsible for the formation of copper nanoparticles. The results of the reaction of the extract against Cu2+ ions were confirmed using UV-Vis Spectrophotometer, Fourier Transform Infrared (FTIR), X-Ray Diffraction (XRD), Scanning Electron Microscope-Energy Dispersive X-Ray(SEM-EDX) and Transmission Electron Microscopy (TEM). The obtained copper nanoparticles showed good antimicrobial activity against Staphylococcus aureus bacteria, Escherichia coli bacteria and Candida albicans fungi. The antibacterial and antifungal activity of the inhibitory zone at a concentration of 20% was categorized as strong (intermediate) and the antifungal was categorized as intermediate. Keywords: Copper nanoparticles, antimicrobial activity, aqueous extract, Polyalthia longifolia.