Electronic Theses and Dissertation

ABSTRAK
Permasalahan gigi dan mulut yang diderita oleh setengah populasi penduduk dunia, yaitu sekitar 3,58 miliar jiwa adalah karies gigi yang dapat disebabkan oleh mikroorganisme. Salah satu bakteri penyebab karies gigi adalah bakteri Streptococcus mutans. Penelitian ini bertujuan untuk mengisolasi dan menguji aktivitas antibakteri isolat bakteri endofit dari daun cengkeh (Syzygium aromaticum L). Isolasi bakteri endofit dilakukan dengan menggunakan metode tempel, dimurnikan dengan metode kuadran T, skrining antagonis dengan metode cross streak, modifikasi cross streak dan cork bore, ekstraksi dengan metode partisi bertingkat dan pengujian aktivitas antibakteri dengan metode difusi cakram. Hasil penelitian ini adalah isolat bakteri endofit yang diperoleh sebanyak 7 isolat yang terdiri dari isolat IDC 1, IDC 2, IDC 3, IDC 4, IDC 5, IDC 6 dan IDC 7. Hasil skrining aktivitas antagonis menunjukkan bahwa IDC 5 mampu memberikan zona hambat terbesar terhadap pertumbuhan bakteri Streptococcus mutans. Hasil uji aktivitas antibakteri dari fraksi n-heksan, etil asetat dan metanol IDC 5 menunjukkan bahwa tidak adanya aktivitas antibakteri terhadap bakteri Streptococcus mutans. Hasil skrining fitokimia dari masing-masing fraksi memberikan hasil negatif terhadap uji alkaloid, flavonoid, fenol, steroid, triterpenoid, saponin dan tanin. Kata kunci: aktivitas antibakteri, bakteri endofit, skrining fitokimia, Streptococcus mutans, Syzygium aromaticum L

ABSTRACT Dental and oral problems suffered by half the world's population, which is around 3.58 billion people are dental caries which can be caused by microorganisms. One of the bacteria that causes dental caries is Streptococcus mutans bacteria. This study aimed to isolate and test the antibacterial activity of endophytic bacterial isolates from clove leaves (Syzygium aromaticum L). Isolation of endophytic bacteria was carried out using the patch method, purification by the T quadrant method, screening of antagonists by the cross streak method, modification of the cross streak and cork bore, extraction by the stratified partition method, and testing of antibacterial activity by the disc diffusion method. The results of this study were isolates of endophytic bacteria obtained as many as 7 isolates consisting of isolates IDC 1, IDC 2, IDC 3, IDC 4, IDC 5, IDC 6, and IDC 7. The results of screening antagonist activity showed that IDC 5 was able to provide the largest inhibition zone. against the growth of Streptococcus mutans bacteria. The results of the antibacterial activity test of the n-hexane, ethyl acetate, and methanol fractions of IDC 5 showed that there was no antibacterial activity against Streptococcus mutans bacteria. The results of phytochemical screening of each fraction gave negative results for the test for alkaloids, flavonoids, phenols, steroids, triterpenoids, saponins, and tannins. Keywords: antibacterial activity, endophytic bacteria, phytochemical screening, Streptococcus mutans, Syzygium aromaticum L.