Electronic Theses and Dissertation

Alpha smooth muscle actin (αSMA) merupakan protein yang berperan dalam proses kontraktilitas sel-sel otot polos pada jaringan seperti testis. Sebaran dan ekspresi αSMA pada testis menjadi penanda perkembangan testis berdasarkan umur. Penelitian ini bertujuan mengetahui sebaran dan ekspresi αSMA pada jaringan testis kelinci lokal dengan tingkatan umur berbeda menggunakan teknik imunohistokimia (IHK). Sampel yang digunakan adalah 12 testis kelinci lokal yang dikoleksi dari 12 ekor kelinci lokal jantan yang dibagi menjadi empat kelompok, yaitu kelinci umur 2 bulan (K-I), 6 bulan (K-II), 1 tahun (K-III), dan 2 tahun (K-IV), setiap kelompok terdiri atas tiga ekor kelinci (n=3). Sampel diproses menjadi preparat histologi dan diwarnai dengan pewarnaan IHK metode avidin biotin complex peroxidase (ABC method). Sebaran αSMA dianalisis secara deskriptif, sedangkan perbedaan ekspresi αSMA dinilai dengan pemberian skor intensitas (intensity score) lalu hasilnya dianalisis menggunakan uji Kruskal-Wallis dan uji lanjut Mann Whitney U Test. Hasil penelitian menunjukkan bahwa αSMA terdeteksi positif pada buluh darah, jaringan ikat, dan sel myoid peritubular. Berdasarkan uji statistik terdapat perbedaan nyata ekspresi αSMA pada sel myoid peritubular (P0,05) antara kelompok umur K-I dengan K-IV, K-II dengan K-III dan K-IV. Kesimpulan penelitian ini adalah sebaran αSMA pada jaringan testis ditemukan pada buluh darah, sel myoid peritubular, dan jaringan ikat dengan ekspresi paling kuat pada sel myoid peritubular testis kelinci produktif (umur 1 tahun).

Kata kunci: Buluh darah, sel myoid peritubular, jaringan ikat, imunoreaktivitas

Alpha smooth muscle actin (αSMA) is a protein that plays a role in the process of contractility of smooth muscle cells in tissues such as the testes. The distribution and expression of αSMA in the testicles is a marker of testicular development based on age. This study aims to determine the distribution and expression of αSMA in local rabbit testicular tissue with different age levels using immunohistochemistry (IHC) techniques. The samples used were 12 local rabbit testicles collected from 12 male local rabbits divided into four groups, namely rabbits aged 2 months (K-I), 6 months (K-II), 1 year (K-III), and 2 years (K-IV), each group consisting of three rabbits (n=3). The sample was processed into histological preparations and stained with IHC staining of the avidin biotin complex peroxidase (ABC method) method. The distribution of αSMA was analyzed descriptively, while the difference in αSMA expression was assessed by giving an intensity score and then the results were analyzed using the Kruskal-Wallis test and the Mann Whitney U Test. The results showed that αSMA was detected positively in blood vessels, connective tissue, and peritubular myoid cells. Based on statistical tests, there were significant differences in αSMA expression in peritubular myoid cells (P0.05) between the K-I and K-IV, K-II and K-IV age groups. The conclusion of this study was that the distribution of αSMA in testicular tissue was found in blood vessels, peritubular myoid cells, and connective tissue with the strongest expression in peritubular myoid cells of productive rabbit testicles (1 year old). Keywords: Blood vessels, peritubular myoid cells, connective tissue, immunoreactivity