Electronic Theses and Dissertation

ABSTRAK

Flavonoid adalah senyawa aktif daun malaka. Flavonoid bermanfaat sebagai aktivitas sitotoksik, antivirus, antifungi, antioksidan, anti-inflamasi, antiproliferasi, antikarsinogenik, antibakteri, antialergi, analgesik, dan antipiretik. Staphylococcus aureus adalah salah satu dari bakteri Gram positif yang berdiameter 0,7-1,2 m, berbentuk coccus, fakultatif anaerob, tidak berspora, dan tidak bergerak. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri senyawa flavonoid daun malaka (Phyllanthus emblica) terhadap pertumbuhan bakteri S. aureus dam mengetahui pada konsentrasi senyawa flavonoid daun malaka (P. emblica) yang dapat menghambat pertumbuhan bakteri S. aureus. Dengan metode Experimental Lab, terdiri dari 6 perlakuan, 4 konsentrasi (10%, 20%, 40%, 80%) senyawa flavonoid daun malaka dan 2 kontrol (kontrol positif dengan kloramfenikol dan kontrol negatif dengan aquadest steril) masing-masing 3 kali pengulangan. Rata-rata pengukuran zona hambat konsentrasi 10% sebesar 10,60 mm, konsentrasi 20% sebesar 17,71 mm, konsentrasi 40% sebesar 19,40 mm, konsentrasi 80% sebesar 20,51 mm, sedangkan rata-rata pengukuran zona hambat kontrol positif sebesar 25,21 mm dengan kontrol negatif sebesar 00,00 mm. Dari kesimpulan penelitian, konsentrasi senyawa flavonoid daun malaka (P. emblica) berbanding lurus dengan diameter zona hambat, yaitu semakin besar konsentrasi yanvg digunakan maka semakin besar pula diameter zona hambat yang akan dihasilkan.

Kata kunci: Phyllanthus emblica, Flavonoid, Staphylococcus aureus, Antibakteri, Kloramfenikol.

ABSTRACT Flavonoids are the active compounds of Malacca leaves. Flavonoids are useful as cytotoxic, antiviral, antifungal, antioxidant, anti-inflammatory, antiproliferative, anticarcinogenic, antibacterial, antiallergic, analgesic, and antipyretic activities. Staphylococcus aureus is one of the gram-positive bacteria with a diameter of 0.7-1.2 m, cocci-shaped, facultative anaerobes, non-sporing, and immobile. This research aims to determine the antibacterial activity of flavonoid compounds from malacca leaves (Phyllanthus emblica) against the growth of S. aureus bacteria and to determine the concentration of flavonoid compounds in Malacca leaves (P. emblica) which can inhibit the growth of S. aureus bacteria. With the Experimental Lab method, consisting of 6 treatments, 4 concentrations (10%, 20%, 40%, 80%) flavonoid compounds of malacca leaves and 2 controls (positive control with chloramphenicol and negative control with aquadest) with each 3 repetitions. The average inhibition zone measurement of 10% concentration is 10.60 mm, 20% concentration is 17.71 mm, 40% concentration is 19.40 mm, 80% concentration is 20.51 mm, while the average inhibition zone measurement of control positive is 25.21 mm with a negative control is 00.00 mm. From the results of the research, the concentration of flavonoid compounds in Malacca leaves (P. emblica) was directly proportional to the diameter of the inhibition zone, which is higher the concentration used, the largest diameter of the inhibition zone that would be produced. Keyword: Phyllanthus emblica, Flavonoid, Staphylococcus aureus, Antibacteria, Chloramphenicol