Electronic Theses and Dissertation

ABSTRAK

Faunus ater merupakan salah satu spesies siput air tawar yang banyak dijumpai di Provinsi Aceh dan sering disebut dengan istilah Cue, yang banyak dimanfaatkan sebagai bahan pangan maupun sebagai sumber ekonomi bagi masyarakat sekitar. Penelitian ini bertujuan untuk mengidentifikasi Shigella sp. pada siput Faunus ater (Cue) di sungai Kawasan Lhoong Aceh Besar. Metode penelitian dilakukan dengan menggunakan siput Faunus ater sebagai sampel pemeriksaan laboratorium yang meliputi isolasi dalam media NB, penanaman pada media SSA, pewarnaan Gram, serta uji biokimia dengan total tiga sampel siput Faunus ater untuk dieksplorasi. Hasil pada media NB menunjukan adanya pertumbuhan bakteri sebagai ciri bakteri anaerob fakultatif. Hasil penanaman pada media SSA menunjukan dua koloni yang berbeda, yaitu koloni bakteri berwarna bening dengan titik hitam di tengah dan koloni berwarna bening tanpa titik hitam di tengah. Secara mikroskopis koloni bakteri yang diuji memperlihatkan bakteri berbentuk basil Gram negatif. Hasil uji biokimia menunjukkan dua sampel sungai teridentifikasi Shigella sonnei. Dapat disimpulkan bahwa, pada sungai Kawasan Lhoong terdapat dua sampel Faunus ater positif teridentifikasi Shigella sonnei.

Kata kunci: Faunus ater, Shigella sp., Shigella sonnei

ABSTRACT Faunus ater is one of the species of freshwater snail that are often found in Aceh Province and often referred to as Cue, which is widely used as food and as an economic source for the surrounding community. This study aims to identify the Shigella sp. in the Faunus ater (Cue) snail in the Lhoong Aceh Besar river. The research method was done by using Faunus ater snails as samples for laboratory examination which included isolation in NB media, planting on SSA media, Gram staining, and biochemical tests with a total of three samples of Faunus ater snails to be explored. The results on NB media showed the presence of bacterial growth as a characteristic of facultative anaerobic bacteria. The results of planting on SSA media showed two different colonies, namely a clear bacterial colony with a black dot in the middle and a clear colony without a black dot in the middle. Microscopically the tested bacterial colonies showed that the bacteria were in the form of Gram-negative bacilli. The results of the biochemical test showed that two river samples were identified as Shigella sonnei. It can be concluded that, in the river of the Lhoong area there were two samples of Faunus ater which were positively identified with Shigella sonnei. Keywords: Faunus ater, Shigella sp., Shigella sonnei