Electronic Theses and Dissertation

Siput Faunus ater merupakan siput dari kelas Gastropoda yang hidup di perairan air tawar yang memperoleh makanannya secara menyaring (filter feeder) substrat lumpur yang terdapat bahan organik di habitatnya. Penelitian ini bertujuan untuk mengidentifikasi Staphylococcus sp. pada siput Faunus ater di kawasan Lhoong, Aceh Besar. Metode penelitian menggunakan rancangan Simple Random Sampling yang sampel diambil secara acak dari 3 sungai dengan masing-masing 3 ekor per sungai. Sebagai perlakuan setiap sungai diambil 3 ekor siput Faunus ater disatukan menjadi 1 sampel, kemudian diinokulasikan ke dalam media Nutrient Broth (NB) dan dilanjutkan dalam media Manitol Salt Agar (MSA) untuk pengamatan morfologi secara makroskopis dan pewarnaan Gram sebagai pengamatan morfologi mikroskopis serta uji biokimia untuk identifikasi spesies bakteri. Hasil pengamatan menunjukkan adanya pertumbuhan bakteri dalam media NB dan media MSA teramati morfologi koloni spesifik bakteri Staphylococcus sp. serta hasil pewarnaan Gram menunjukkan bakteri Gram positif. Hasil identifikasi bakteri terlihat adanya reaksi positif katalase dan reaksi positif negatif pada uji gula-gula. Dapat disimpulkan bahwa berdasarkan hasil isolasi dan identifikasi bakteri pada siput Faunus ater atau Cue di kawasan Lhoong Aceh Besar tercemar bakteri Staphylococcus epidermidis.

Kata kunci : Kawasan Lhoong, Siput Faunus ater, Staphylococcus sp.

Faunus ater are snails of the Gastropoda class that live in freshwater waters that obtain their food by filtering (filter feeder) mud substrates that contain organic matter in the snuffed out. This study aims to identify Staphylococcus sp. on the Faunus ater snail in Lhoong, Aceh Besar. The research method uses a Simple Random Sampling design in which samples are taken randomly from 3 rivers with 3 heads each per river. As the treatment of each river was taken 3 snails Faunus ater put together into 1 sample, then inoculated into the media Nutrient Broth (NB) and continued in Mannitol Salt Agar (MSA) media for macroscopic morphological observation and Gram staining as microscopic morphological observations as well as biochemical tests for the identification of bacterial species. The observations showed the growth of bacteria in nb media and MSA media observed morphology of specific colonies of Staphylococcus bacteria and Gram staining results showed Gram positive bacteria. The results of bacterial identification showed a positive reaction of catalase and a negative positive reaction on the confectionery test. It can be concluded that based on the results of isolation and identification of bacteria in Faunus ater snails or Cue in the Lhoong Aceh Besar area contaminated with Staphylococcus epidermidis. Keywords : Lhoong area, Faunus ater Snail, Staphylococcus sp.