Electronic Theses and Dissertation

ABSTRAK

Faunus ater merupakan siput yang berasal dari famili Pachychilidae, kelas Gastropoda, dan termasuk ke dalam filum Mollusca. Faunus ater memenuhi nutrisinya dengan cara menyaring (filter feeder) substrat sehingga memiliki potensi terjadinya bioakumulasi dengan mikroba patogen. Penelitian ini bertujuan untuk mengidentifikasi keberadaan bakteri Salmonella sp. pada siput Faunus ater di Sungai Kawasan Leupung Aceh Besar. Metode penelitian dilakukan secara observasi lapangan dan analisis laboratorium menggunakan metode Carter dengan mengoleksi sebanyak 9 siput Faunus ater yang dijadikan sebagai 3 sampel penelitian dan memecahkan cangkangnya kemudian diinokulasikan ke dalam media Selenite Cystine Broth (SCB), dilanjutkan pembiakan di dalam media Salmonella Shigella Agar (SSA) untuk mengamati morfologi koloni secara makroskopis dan pewarnaan Gram untuk mengamati morfologi secara mikroskopis, serta identifikasi spesies bakteri dengan uji biokimia. Hasil penelitian menunjukkan adanya reaksi pada media SCB dan pertumbuhan koloni pada media SSA, pewarnaan Gram menunjukkan bakteri Gram negatif berbentuk batang panjang berwarna merah, reaksi biokimia menunjukkan adanya bakteri Salmonella sp. Dapat disimpulkan bahwa pada siput Faunus ater di Sungai Kawasan Leupung Aceh Besar teridentifikasi adanya bakteri Salmonella spesies Salmonella Paratyphi A dan Salmonella typical.

Kata kunci : Siput Faunus ater, Sungai Kawasan Leupung, Salmonella sp.

ABSTRACT Faunus ater is a snail that belongs to the family Pachychilidae, a class of Gastropods, and belongs to the phylum Mollusca. Faunus ater meets its nutrients by filtering (filter feeder) substrate so that it has the potential for bioaccumulation with pathogenic microbes. This study aims to identify the presence of Salmonella sp. bacteria. on the Faunus ater snail in the Leupung Area River of Aceh Besar. The research method was carried out by field observation and laboratory analysis using the Carter method by collecting as many as 9 Faunus ater snails which were used as 3 research samples and breaking their shells then inoculated into Selenite Cystine Broth (SCB) media, followed by breeding in Salmonella Shigella Agar (SSA) media to observe colony morphology macroscopically and Gram staining to observe morphology microscopically, as well as identification of bacterial species by biochemical tests. The results showed a reaction in the SCB media and colony growth in the SSA media, Gram staining indicates gram negative bacteria in the form of a long red rod, biochemical reactions indicate the presence of Salmonella sp. bacteria. It can be concluded that in the Faunus ater snail in the River Leupung Area of Aceh Besar identified the presence of Salmonella bacteria species Salmonella Paratyphi A and Salmonella typical. Keywords : Faunus ater snail, Leupung area river, Salmonella sp.