Electronic Theses and Dissertation

Siput Faunus ater termasuk filum Mollusca, yaitu hewan yang tidak bertulang belakang dan tergolong dalam kelas Gastropoda. Cirinya bertubuh lunak dan dilindungi oleh cangkang tunggal. Penelitian ini bertujuan untuk mengidentifikasi bakteri Vibrio sp. pada siput Faunus ater di kawasan Krueng Reuleung, Leupueng, Aceh Besar. Metode penelitian dilakukan secara observasi dan analisis laboratorium menggunakan metode carter dengan mengkoleksi sebanyak 9 siput Faunus ater yang dijadikan sebagai 3 sampel perlakuan dengan cara memecahkan cangkangnya dan diinokulasikan ke dalam media Alkaline Peptone Water (APW), dilanjutkan pembiakan di dalam media Thiosulfate Citrate Bilt Salt Sucrose (TCBS) untuk mengamati morfologi secara makroskopis dan pewarnaan Gram untuk mengamati morfologi secara mikroskopis serta identifikasi bakteri dengan uji Biokimia. Hasil penelitian menunjukkan adanya reaksi pada media APW dan pertumbuhan koloni pada media TCBS. Pewarnaan Gram menunjukkan bakteri Gram negatif berbentuk spiral dan berwarna merah, reaksi Biokimia menunjukkan adanya bakteri Vibrio spesies Vibrio Parahaemolyticus. Dapat disimpulkan bahwa dari ketiga sampel Faunus ater yang diambil di Kawasan Krueng Reuleung, Leupueng, Aceh Besar teridentifikasi bakteri Vibrio parahaemolyticus.

Kata kunci: Faunus ater, Krueng Reuleung, Vibrio sp.

Faunus ater snail belongs to the phylum Mollusca, which is an animal that does not have a backbone and belongs to the class Gastropoda. Characteristics of soft body and protected by a single shell. This study aims to identify the bacteria Vibrio sp. on the Faunus ater snail in the Krueng Reuleung area, Leupueng, Aceh Besar. The research method was carried out by observation and laboratory analysis using the charter method by collecting as many as 9 Faunus ater snails which were used as 3 treatment samples by breaking their shells and inoculated into Alkaline Peptone Water (APW), media, followed by culturing in Thiosulfate Citrate Bilt Salt Sucrose (TCBS) media to observe macroscopic morphology and Gram staining to observe microscopic morphology and identification of bacteria by biochemical tests. The results showed that there was a reaction on APW media and colony growth on TCBS media. Gram staining shows spiral shaped and red Gram negative bacteria, Biochemical reactions show the presence of Vibrio bacteria species Vibrio Parahaemolyticus. It can be concluded that from the three samples of Faunus ater taken in the Krueng Reuleung area, Leupueng, Aceh Besar, Vibrio parahaemolyticus was identified. Keywords: Faunus ater, Krueng Reuleung, Vibrio sp.