Electronic Theses and Dissertation

ABSTRAK
Salmonella sp. merupakan bakteri patogen yang dapat menyebabkan penyakit Salmonellosis pada hewan maupun manusia. Penularan bakteri Salmonella sp. pada ayam dapat melalui pakan, air, hewan pengerat, serangga, peternak, dan peralatan kandang. Penelitian ini bertujuan melihat keberadaan Salmonella sp. pada nipple drinker dan air tandon di kandang ayam broiler dengan sistem close house pada peternakan Japfa Iee Suum Aceh Besar. Sampel penelitian ini berupa swab pada ujung nipple drinker dan air tandon. Jumlah sampel swab pada ujung nipple drinker sebanyak 21 sampel dan air tandon sebanyak 2 sampel. Penelitian ini menggunakan metode Cross sectional yang dimulai dengan pengambilan sampel swab diujung nipple drinker dan penampungan air tandon dengan tabung reaksi steril kemudian dimasukkan kedalam media Selenite Cystine Borth (SCB), perbandingan sampel air dan larutan SCB 1:9. Selajutnya dilakukan penanaman pada media Salmonella Shigella Agar (SSA). Koloni yang diduga Salmonella sp. kemudian ditanam pada NA miring dan dilajutkan dengan pewarnaan Gram. Setelah itu untuk mengetahui jenis spesies bakteri Salmonella sp. dilakukan penanaman ke dalam media indol, Methyl Red (MR), Voges Proskauer (VP), Sulfid Indol Motility (SIM), Simmon Citrate Agar (SCA) (IMVIC), Triple Sugar Iron Agar (TSIA) dan gula-gula (glukosa, sukrosa, laktosa, manitol, dan maltosa). Data hasil penelitian dianalisis secara deskriptif, ditampilkan dalam bentuk gambar dan tabel. Hasil isolasi dan identifikasi dari 2 sampel air tandon dan 21 sampel nipple drinker, diperoleh hasil bahwa pada sampel air tandon tidak ditemukan Salmonella sp. dan pada sampel nipple drinker 8(38,1%) sampel positif Salmonella sp. yang terdiri dari 2 spesies Salmonella yaitu 3(14,2%) isolat Salmonella thypimurium, dan 5(23,8%) isolat Salmonella enteretidis.

Kata kunci : Salmonella sp., Close house, Nipple drinker, Salmonellosis.

ABSTRACT Salmonella sp. is a pathogenic bacterium that can cause salmonellosis in animals and humans. Salmonella sp. in chickens can be through feed, water, rodents, insects, breeders, and cage equipment. This study aims to determine the presence of Salmonella sp. on nipple drinkers and water reservoirs in a broiler chicken coop with a close house system at the Japfa Iee Suum farm in Aceh Besar. The sample of this research is a swab at the tip of the nipple drinker and water tandon. The number of swab samples at the tip of the nipple drinker was 21 samples and the reservoir water was 2 samples. This study used a cross sectional method, starting with taking a swab sample at the tip of the nipple drinker and holding the reservoir water with a sterile test tube and then inserting it into Selenite Cystine Borth (SCB) media, the ratio of water samples and SCB solution 1: 9. Shigella Agar (SSA). Colonies suspected of Salmonella sp. then planted in NA oblique and followed by Gram staining. After that to determine the type of bacterial species Salmonella sp. were planted in indole, Methyl Red (MR), Voges Proskauer (VP), Sulfid Indole Motility (SIM), Simmon Citrate Agar (SCA) (IMVIC), Triple Sugar Iron Agar (TSIA) and sugars (glucose, sucrose, lactose, mannitol, and maltose). The research data were analyzed descriptively, displayed in the form of pictures and tables. The results of the isolation and identification of 2 samples of reservoir water and 21 samples of nipple drinkers, showed that Salmonella sp. and 8(38.1%) nipple drinker samples were positive for Salmonella sp. consisting of 2 species of Salmonella, namely 3 (14.2%) isolates of Salmonella thypimurium, and 5 (23.8%) isolates of Salmonella enteretidis Keywords : Salmonella sp., Close house, Nipple drinker, Salmonellosis