Electronic Theses and Dissertation

Aktivitas sitotoksik dan antioksidan ekstrak n-heksana daun sembung (Blumea balsamifera L.) dan 10 kelompok fraksi telah diuji dengan menggunakan metode Brine Shrimp Lethality Test (BSLT) dan 1,1-diphenyl-2-pycrixlhidrazil (DPPH). Ekstrak n-heksana daun sembung dipisahkan komponen kimianya dengan menggunakan kromatografi kolom dan diperoleh 10 kelompok fraksi (BBH 1-BBH 10) dan diuji aktivitas sitotoksik dan antioksidan. Keseluruhan fraksi dikategorikan sampel yang sangat toksik. Fraksi BBH 8 menunjukkan aktivitas sitotoksik tertinggi dengan nilai LC50 sebesar 2,00 ppm dan memiliki aktivitas antioksidan yang sedang dan lemah dengan aktivitas antioksidan tertinggi pada fraksi BBH 8 nilai IC50 sebesar 113,69 ppm. Isolasi lebih lanjut dilakukan terhadap fraksi BBH 8 memperoleh 10 subfraksi gabungan (BBH 8.1–BBH 8.10). Subfraksi BBH 8.8 direkromatografi, hasil rekromatografi diperoleh 48 subfraksi. Subfraksi BBH 8.8.5 diKLT 2 dimensi mengandung senyawa terpenoid yang ditandai timbulnya warna ungu setelah disemprot dengan vanillin sulfat pada plat KLT. Hasil karakterisasi GC-MS isolat murni memiliki satu senyawa yang dominan yaitu Cryptomeridiol (7-(2-Hydroxypropan-2-yl)-1,4a- dimethyldecahydronaphthalen-1-ol) serta memiliki aktivitas sitotoksik yang kuat dengan nilai LC50 25,03 ppm dan memiliki aktivitas antioksidan lemah dengan nilai IC50 293,10 ppm.

Kata kunci: Daun sembung (Blumea balsamifera L.), ekstrak n-heksana, sitotoksik, antioksidan, Brine Shrimp Lethality Test (BSLT) dan 1,1-diphenyl-2-pycrixlhidrazil (DPPH).

Cytotoxic and antioxidant activity of n-hexane extract of sembung leaf (Blumea balsamifera L.) and 10 groups of fractions were tested using the Brine Shrimp Lethality Test (BSLT) and 1,1-diphenyl-2-pycrixlhydrazil (DPPH) methods. The chemical components of the n-hexane extract of Sembung leaves were separated using column chromatography and obtained 10 groups of fractions (BBH 1-BBH 10) and tested for cytotoxic and antioxidant activity. All fractions were categorized as very toxic samples. BBH 8 fraction showed the highest cytotoxic activity with an LC50 value of 2.00 ppm and had moderate and weak antioxidant activity with the highest antioxidant activity at IC50 fraction 8 with an IC50 value of 113.69 ppm. Further isolation was carried out on the BBH 8 fraction to obtain 10 combined subfractions (BBH 8.1–BBH 8.10). BBH 8.8 subfraction was rechromatographed, the rechromatography results obtained 48 subfractions. The BBH 8.8.5 subfraction in 2-dimensional TLC contains terpenoid compounds which are characterized by the appearance of a purple color after being sprayed with vanillin sulfate on the TLC plate. The results of GC-MS characterization of pure isolates had one dominant compound, namely Cryptomeridiol (7-(2-Hydroxypropan-2-yl)-1,4a-dimethyldecahydronaphthalen-1-ol) and had strong cytotoxic activity with an LC50 value of 25.03 ppm. and has weak antioxidant activity with IC50 value of 293.10 ppm. Keywords: Sembung leaf (Blumea balsamifera L.), n-hexane extract, cytotoxic, antioxidant, Brine Shrimp Lethality Test (BSLT) and 1,1-diphenyl-2-pycrixlhidrazil (DPPH).