Electronic Theses and Dissertation

Puyuh (coturnix-coturnix japonica) adalah salah satu habitat utama bakteri Shigella sp. yang menyebabkan terjadinya shigellosis. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi Shigella sp. yang terdapat pada kloaka puyuh. Penelitian ini dilakukan berdasarkan cross sectional study yang dikerjakan sesuai SNI 01-2336-1991. Koleksi sampel diambil dari 15 ekor puyuh dengan gejala diare, dehidrasi, anoreksia, puyuh yang tampak kurus dan lemas serta suka mengisolasi diri di sudut kandang. Data hasil penelitian kemudian dianalisis secara deskriptif yang ditampilkan dalam bentuk gambar dan juga tabel. Hasil penelitian diperoleh 6 sampel yang menunjukkan positif Shigella sp. (39,6%) dan 7 sampel menunjukkan negatif Shigella sp. (59,4%). Oleh karena itu, dapat disimpulkan bahwa hasil isolasi dan identifikasi Shigella sp. pada kloaka puyuh (Coturnix-coturnix japonica) ditemukan Shigella flexneri (3 sampel), Shigella dysenteriae (1 sampel) dan Shigella sonnei (2 sampel) dan sebanyak 39,6% diare pada puyuh disebabkan oleh Shigella sp.

Kata kunci : Foodborne disease, Shigella sp., shigellosis, puyuh (Coturnix-coturnix japonica)

Quail (Coturnix-coturnix japonica) is one of the main habitats for Shigella sp. which causes shigellosis. This study aims to isolate and identify Shigella sp. found in quail cloaca. This research was conducted based on a cross sectional study carried out according to SNI 01-2336-1991. The sample collection was taken from 15 quail with symptoms of diarrhea, dehydration, anorexia, quail that looked thin and weak and liked to isolate themselves in the corner of the cage. The research data were then analyzed descriptively which were displayed in the form of pictures and the tables. The result showed that 6 samples were positive Shigella sp. (39,6%) and 7 samples showed negative Shigella sp. (59,4%). Therefore, it can be concluded that the result of isolation and identification of Shigella sp. in the cloaca of quail (Coturnix-coturnix japonica) Shigella flexneri (3 samples), Shigella dysenteriae (1 samples), and Shigella sonnei (2 samples) were found, and 39,6% diarrhea in quail caused by Shigella sp. Keyword : foodborne disease, Shigella sp., shigellosis, quail (Coturnix-coturnix japonica).