Electronic Theses and Dissertation

Escherichia coli merupakan bakteri patogen penyebab infeksi pada manusia dan hewan yang dilaporkan telah mengalami peningkatan resistensi terhadap antibiotik. Situasi ini memicu perlunya pemanfaatan agen antibakteri alternatif dari bahan alam. Salah satunya adalah tumbuhan sarang semut (Hydnophytum formicarum) asal Aceh. Penelitian ini bertujuan untuk mengevaluasi aktivitas antibakteri ekstrak metanol umbi Hydnophytum formicarum asal Aceh terhadap pertumbuhan Escherichia coli. Penelitian dilaksanakan secara eksperimental menggunakan metode rancangan acak lengkap yang terdiri atas enam perlakuan, yaitu ekstrak pada konsentrasi 6,25%, 12,5%, 25%, dan 50%, kontrol positif ciprofloxacin, serta kontrol negatif CMC 1%. Uji aktivitas antibakteri dilaksanakan dengan teknik Kirby–Bauer di atas media Mueller Hinton Agar (MHA) dengan tiga kali ulangan. Data dianalisis menggunakan One Way ANOVA menggunakan aplikasi SPSS dan dilanjutkan dengan uji Duncan Multiple Range Test (DMRT). Hasil penelitian menunjukkan bahwa ekstrak metanol Hydnophytum formicarum pada konsentrasi 6,25%, 12,5%, dan 25% tidak menunjukkan zona hambat terhadap Escherichia coli. Aktivitas antibakteri mulai teramati pada konsentrasi 50% dengan rata-rata diameter zona hambat sebesar 9,8 ± 0,346 mm dan tergolong kategori sedang. Hasil analisis statistik menunjukkan adanya perbedaan nyata antar perlakuan (P

Escherichia coli is a pathogenic microorganism responsible for inducing infections across both human and animal hosts and has been reported to exhibit increased resistance to antibiotics. This condition encourages the exploration of alternative antibacterial agents sourced from natural sources. One such source is the ant-plant (Hydnophytum formicarum) from Aceh. This study aimed to evaluate the antibacterial activity of the methanol extract of Hydnophytum formicarum tubers from Aceh against the growth of Escherichia coli. The research was conducted experimentally using a completely randomized design method consisting of six treatments, namely extract concentrations of 6.25%, 12.5%, 25%, and 50%, a positive control of ciprofloxacin, and a negative control of 1% CMC. Antibacterial activity testing was performed using the Kirby–Bauer technique on Mueller Hinton Agar (MHA) media with three replications. Data were analyzed using one-way ANOVA with SPSS software and followed by Duncan’s Multiple Range Test (DMRT). The results showed that the methanol extract of Hydnophytum formicarum at concentrations of 6.25%, 12.5%, and 25% did not produce inhibition zones against Escherichia coli. Antibacterial activity was first observed at a concentration of 50%, with an average inhibition zone diameter of 9.8 ± 0.346 mm and categorized as moderate. The results of the statistical analysis indicated a significant difference among treatments (P