Electronic Theses and Dissertation

Trypanosoma evansi merupakan protozoa darah yang menyebabkan penyakit surra. surra dapat memengaruhi profil darah hewan, penyakit ini menimbulkan kerugian ekonomi yang tidak sedikit. namun pengobatan surra belum optimal karena obatnya susah didapat dan adanya galur resistensi obat. penelitian ini bertujuan untuk mengetahui efek ekstrak daun kecombrang (etlingera elatior) dalam mempertahankan profil darah tikus yang diinfeksikan t. evansi. penelitian ini menggunakan 25 ekor tikus putih yang dibagi kedalam 5 kelompok dan masing-masing kelompok terdiri dari 5 ekor tikus. kelompok kontrol negatif (k1) tanpa infeksi t.evansi dan tanpa ekstrak ekstrak daun kecombrang, kelompok kontrol positif (k2) diinfeksikan t. evansi tanpa diberikan ekstrak daun kecombrang, kelompok perlakuan k3, k4, dan k5 diinfeksikan t. evansi dan diberi ekstrak daun kecombrang dengan dosis 300, 600, dan 1200 mg/kg berat badan. infeksi t. evansi dilakukan secara intraperitoneal sedangkan ekstrak diberikan secara oral selama 4 hari berturut-turut. pengambilan darah dilakukan pada hari kelima setelah perlakuan. hasil penelitian menunjukkan bahwa rata-rata ± sd nilai jumlah eritrosit, jumlah leukosit, kadar hemaglobin dan kadar hematokrit meningkat sedangkan jumlah sel-sel leukosit menurun setelah pemberian ekstrak daun kecombrang. berdasarkan hasil penelitian dapat disimpulkan bahwa pemberian ekstrak daun kecombrang (e. elatior) dosis 300mg/kg tidak mampu mempertahankan profil darah tikus, sedangkan 600 dan 1200 mg/kg bb pada tikus putih yang telah diinfeksikan t. evansi mampu mempertahankan bahkan meningkatkan jumlah eritrosit, kadar hematokrit dan kadar hemaglobin mendekati nilai normal serta mempertahankan jumlah leukosit dalam kisaran normal.

Trypanosoma evansi is a blood protozoan that causes surra disease. . Surra can affect the blood profile of animals, and this disease causes significant economic losses. However, the treatment of surra is not yet optimal because the medicine is hard to obtain and there are drug-resistant strains. This study aims to examine the effect of torch ginger leaves extract (Etlingera elatior) in maintaining the blood profile of rats infected with T. evansi. This study used 25 white rats divided into 5 groups, with each group consisting of 5 rats. The negative control group (K1) was neither infected with T. evansi nor given the torch ginger leaf extract, the positive control group (K2) was infected with T. evansi but not given the torch ginger leaf extract, and the treatment groups K3, K4, and K5 were infected with T. evansi and given torch ginger leaf extract at doses of 300, 600, and 1200 mg/kg body weight, respectively. T. evansi infection was carried out intraperitoneally, while the extract was administered orally for 4 consecutive days. Blood sampling was conducted on the fifth day. Blood samples were taken on the fifth day after treatment. The study results showed that the mean ± SD values of red blood cell count, white blood cell count, hemoglobin level, and hematocrit level increased, while the number of leukocyte cells decreased after administration of kecombrang leaf extract. Based on the study results, it can be concluded that administration of kecombrang leaf extract (E. elatior) at a dose of 300 mg/kg was not able to maintain the blood profile in rats, whereas doses of 600 and 1200 mg/kg in T. evansi-infected white rats were able to maintain and even increase red blood cell count, hematocrit levels, and hemoglobin levels approaching normal values, as well as maintain leukocyte counts within the normal range.