Electronic Theses and Dissertation

Karies gigi merupakan masalah kesehatan umum akibat biofilm mikroba di rongga mulut, terutama streptococcus mutans. minyak nilam (pogostemon cablin benth), dengan kandungan aktif seperti patchouli alcohol yang terbukti memiliki aktivitas antimikroba. penelitian ini bertujuan mengevaluasi aktivitas antibiofilm minyak nilam terhadap biofilm s. mutans pada tikus wistar (rattus norvegicus) yang diinduksi karies. desain penelitian menggunakan metode pretest-posttest only control group dengan tiga kelompok: kontrol negatif (tanpa perlakuan), kontrol positif (diberikan amoksisilin), dan perlakuan (diberikan minyak nilam). analisis data melibatkan uji statistik paired t-test untuk menilai perbedaan sebelum dan setelah dilakukan manipulasi karies. hasil penelitian menunjukkan adanya peningkatan massa biofilm setelah manipulasi karies, tetapi tidak terdapat perbedaan signifikan secara statistik. uji one way anova untuk melihat pengaruh pemberian minyak nilam terhadap massa biofilm s. mutans. hasilnya pemberian minyak nilam menurunkan jumlah biofilm secara deskriptif, namun tidak menunjukkan pengaruh signifikan secara statistik terhadap penghambatan biofilm s. mutans. kesimpulan dari penelitian ini adalah massa biofilm mengalami peningkatan setelah dilakukan manipulasi karies namun secara statistik tidak terdapat perbedaan yang signifikan. kemampuan minyak nilam (pogostemon cablin benth) mampu menurunkan jumlah massa biofilm s.mutans secara deskriptif walau secara statistik tidak menunjukkan pengaruh yang signifikan dalam menghambat biofilm s. mutans pada gigi tikus rattus norvegicus yang diinduksi karies.

Dental caries is a common health problem caused by microbial biofilms in the oral cavity, especially Streptococcus mutans. Patchouli oil (Pogostemon cablin Benth), with active ingredients such as patchouli alcohol which has been proven to have antimicrobial activity. This study aims to evaluate the anti-biofilm activity of patchouli oil against caries-induced S. mutans biofilms in Wistar rats (Rattus norvegicus). The research design used a pretest-posttest only control group method with three groups: negative control (without treatment), positive control (given amoxicillin), and treatment (given patchouli oil). Data analysis involved the Paired T-Test statistical test to assess differences before and after caries manipulation. The results showed an increase in biofilm mass after caries manipulation, but there was no statistically significant difference. One way ANOVA test to see the effect of giving patchouli oil on S. mutans biofilm mass. The results: The application of patchouli oil decreased the number of biofilms descriptively, but did not show a statistically significant effect on inhibiting S. mutans biofilms. The conclusion of this study was that the biofilm mass increased after caries manipulation, but statistically there was no significant difference. The ability of patchouli oil (Pogostemon cablin Benth) was able to reduce the mass amount of S. mutans biofilm descriptively although statistically it did not show a significant effect in inhibiting S. mutans biofilm on the teeth of Rattus norvegicus mice which were induced by caries