Electronic Theses and Dissertation

Newcastle disease (nd) merupakan peyakit menular yang disebabkan oleh virus yang dapat menyerang unggas, seperti ayam dan itik. saat terinfeksi virus, tubuh host akan mengaktifkan sistem kekebalan bawaan dengan produksi interferon untuk membatasi replikasi virus. viperin merupakan protein antiviral yang distimulasi oleh interferon karena adanya infeksi virus. penelitian ini bertujuan mengetahui ekspresi protein viperin pada proventrikulus ayam kampung dan itik yang diinfeksikan isolat vnd virulen asal aceh. sampel penelitian yang digunakan yaitu jaringan proventrikulus ayam kampung dan itik alabio yang dikoleksi pada hari ke-1, 3, dan 7 pasca infeksi (pi) serta jaringan proventrikulus ayam kampung dan itik yang tidak diinfeksi vnd sebagai kontrol negatif. jaringan proventrikulus diwarnai dengan pewarnaan imunohistokimia menggunakan antibodi anti-viperin ea. derajat imunopositif protein viperin pada jaringan proventrikulus ditentukan dengan kategori sedikit, sedang, dan banyak. hasil penelitian menunjukkan protein viperin terekspresi pada jaringan proventrikulus ayam kampung dan itik perlakuan, sedangkan pada sampel kontrol negatif, protein viperin tidak terekspresi. protein viperin terekspresi pada sitoplasma sel epitel kelenjar. perbedaan derajat imunopositif terhadap protein viperin pada jaringan proventrikulus kedua unggas terdapat pada hari ke-1 pi, yaitu protein viperin terekspresi dengan derajat sedikit pada jaringan proventrikulus ayam kampung, sedangkan pada jaringan proventrikulus itik protein viperin terekspresi dengan derajat sedang. kata kunci: imunohistokimia, newcastle disease, proventrikulus, viperin

Newcastle disease (ND) is an infectious disease caused by a virus that can affect poultry, such as chickens and ducks. When infected with the virus, the host body will activate the innate immune system with the production of interferon to limit virus replication. Viperin is an antiviral protein stimulated by interferon due to viral infection. This study aims to determine the expression of viperin protein in the proventriculus of native chickens and ducks infected with virulent VND virus isolates from Aceh. The research samples used were proventriculus tissues of native chickens and Alabio ducks collected on days 1, 3, and 7 post-infection (pi) and proventriculus tissues of native chickens and ducks that were not infected with VND as negative controls. Proventriculus tissues were stained by immunohistochemical staining using Anti-Viperin Ea antibody. The immunopositive degree of viperin protein in proventriculus tissue was determined with the categories of few, moderate, and many. The results showed that the viperin protein was expressed in the proventriculus tissue of native chickens and treated ducks, while in the negative control sample the viperin protein was not expressed. Viperin protein was expressed in the cytoplasm of glandular epithelial cells. The difference in the degree of immunopositivity to viperin protein in the proventriculus tissue of the two poultry was found on day 1 pi, namely the viperin protein was expressed with a few degree in the proventriculus tissue of native chickens, while in the duck proventriculus tissue the viperin protein was expressed with a moderate degree. Keywords: immunohistochemistry, Newcastle disease, proventriculus, viperin